FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n02_MT_30852.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_30852.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26882147
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG429400.15973426527278495No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAT171850.051.13945145
GCTATAT186300.049.975266145
TAGTGTT183650.035.021572145
CCGTATA51850.034.262596145
TCGTGTT263450.030.358494145
GATCCTG133400.030.2166255
CGTGTAT119900.028.726145145
GGGAAAA267500.028.164026145
CAATTAG127750.025.6493939
TATAGTT211900.024.32997145
GAGCAAT128250.023.684286
GAGTGTT231900.023.66999145
TGTACTC72100.022.136812
TCACGAG124400.022.1027582
GCATTAG73600.021.3751375
TTGTACT73600.021.1103021
TTTGATC222850.021.0629542
ACGAGCA128550.020.8676914
TCCTGGC183300.020.5260817
TGGGATC212550.020.3428962
Produced by FastQC (version 0.11.5)