FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n02_MT_30852.TR1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_30852.TR1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29397764
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG351120.11943765519037434No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTAT196050.037.650543145
GGGAAAA434400.031.230167145
ACGTCCG190450.030.87665145
TCGTGTT213900.030.169601145
AAGAGTT269800.028.326242145
CGTGTAT207650.028.319088145
AGCATTA158500.026.0284864
GTAGATA530550.024.176413145
GTACGTA163250.022.119051145
CGATCTA118400.021.5554394
CATTAGA192100.021.2838636
CTATTAG150500.020.856818
AGTGTAA426750.020.270163145
TGTACGG166600.019.933374145
GCATTAG222350.019.5955855
TAGTTGT132400.018.944669
AGATCTA195550.018.799246145
CCGTATA213550.018.23329145
TTGTACT143250.017.4869081
TGTACTC152900.017.2244432
Produced by FastQC (version 0.11.5)