FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n02_MT_30852.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_30852.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29287209
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG322110.10998316705425908No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTAT194950.039.09079145
GGGAAAA425900.030.645016145
TCGTGTT209750.030.52486145
ACGTCCG191850.028.686329145
CGTGTAT207000.026.937105145
AAGAGTT269750.026.127615145
GTAGATA530750.025.028248145
AGCATTA158150.024.709174
CGATCTA120850.022.196974
CATTAGA182800.020.8998586
CTATTAG149450.020.5187268
AGTGTAA420650.019.978252145
CCGTATA210100.019.878857145
TAGTTGT135700.019.5526879
GTACGTA165550.019.490627145
AGATCTA195800.018.997639145
TGTACGG166550.018.938238145
TTGTACT140450.018.9183581
TGTACTC150150.018.6003462
GCATTAG222600.018.3034085
Produced by FastQC (version 0.11.5)