FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n02_MT_30844.TR4_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_30844.TR4_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25325628
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1216870.4804895657473923No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG372940.14725794756205057No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT309520.12221612036629458No Hit
GGAATATTAACCTGTTTCCCATCGACTACGCCTTTCGGCCTCGCCTTAGG302120.11929417900318207No Hit
GCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCC296020.11688555166331907No Hit
CTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACG293620.11593789500501232No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGATT83200.071.6227346
GATCGAT84150.070.9052055
TCGATTA84950.070.317547
CGATTAG86050.069.5875558
AGATTAT65500.061.663326145
TCGATCG96650.061.600153
CGATCGA100000.059.6715624
AGTGTAT39500.058.01002145
TGTTAAA63700.053.84368145
GCGACTA44000.052.2695432
ATTAGTT45800.051.61372145
TGTTAAT60900.050.246773145
GATCCAA90600.048.4927525
GGGAAAA130600.047.58287145
CCTAACT106350.043.1825522
GTAGATA54100.042.756924145
ATCCAAC109050.042.0804566
GATCCAG166200.041.396885
TTGAACG68950.039.9529767
ATCCAGC173150.039.6487856