FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n02_MT_30844.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_30844.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25181380
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1209110.480160340696181No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG344600.1368471465821174No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT309130.1227613419121589No Hit
GGAATATTAACCTGTTTCCCATCGACTACGCCTTTCGGCCTCGCCTTAGG301790.11984648974758333No Hit
GCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCC295540.11736449710063547No Hit
CTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACG293240.11645112380655866No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAT82150.070.1571355
TCGATTA82450.068.75697
ATCGATT82700.068.72456
CGATTAG83250.068.010318
AGATTAT71150.062.978493145
AGTGTAT37900.061.79339145
TCGATCG95050.060.1132283
CGATCGA100300.057.608664
ATTAGTT48650.057.52851145
TGTTAAA58700.053.608093145
GGGAAAA129550.049.643784145
GATCCAA88150.048.7690935
GCGACTA43000.048.585082
GTAGATA54050.047.48826145
TGTTAAT61500.047.15889145
TTGAACG67250.043.765717
CCTAACT110050.042.2519572
ATCCAAC103450.041.9756436
GATCCAG164250.041.136075
TCAGATT73700.039.1736832
Produced by FastQC (version 0.11.5)