FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n02_MT_30830.TR1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_30830.TR1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24163615
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7999323.3104814821788877No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG377730.156321808636663No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT299760.1240542857515318No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA264140.1093131139525274No Hit
GCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCC262690.10871303817744159No Hit
CTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACG260510.10781085528800223No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT138950.049.9058531
TGTACTC142450.048.3344542
TAGTTGT147100.045.88149
TACTCTA156400.045.061314
GCCTAAC108900.042.8069041
CGTGTAT188250.041.25165145
GTACTCT167600.041.0633743
CGTGTAA45950.040.711872145
TCTAGTT176400.040.1508527
CTCTAGT176100.039.8899236
ACTCTAG176200.039.7882925
ATCGATT90900.035.6485946
CGATTAG86250.035.469488
GGGAAAA255250.031.95756145
CCTAACT157950.030.9135192
CCGTGTT122650.028.73132145
GATCCAG169700.028.495665
CGATCGA119300.027.5316374
TCGATCG110850.027.4774233
GATCCAA112100.026.451635
Produced by FastQC (version 0.11.5)