FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n02_MT_30830.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_30830.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23873795
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7962553.3352678114225243No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG346790.14525968745228818No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT299340.12538433876976826No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA263240.11026315673733482No Hit
GCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCC262350.10989036305287869No Hit
CTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACG260230.10900236011911804No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT139700.052.5371361
TGTACTC141400.051.5115472
TAGTTGT143050.049.8152129
TACTCTA158000.047.0794264
CTCTAGT172500.042.6552356
GTACTCT172150.042.0391163
ACTCTAG175650.042.015955
TCTAGTT176550.041.7999237
CGTGTAT185100.039.643696145
CGTGTAA46050.039.365017145
GCCTAAC106150.039.1191
ATCGATT88000.034.51656
CGATTAG85450.032.8316358
GGGAAAA261350.032.155922145
CCGTGTT124400.029.202322145
GATCCAG167700.028.4450915
CCTAACT156500.027.8599572
GATCCAA111100.027.2757515
TGATCCA299900.026.0847034
TCGATCG111650.025.1351993
Produced by FastQC (version 0.11.5)