FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n02_MT_30786.TR2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_30786.TR2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22956048
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13773375.99988726282503No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA449410.1957697596729193No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT407840.17766124203957057No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG366250.1595440121052195No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA348840.1519599540826888No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA305850.13323286307817445No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA303820.13234856452643765No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA254170.11072027728814646No Hit
GCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCC244780.1066298519675512No Hit
CGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241930.10538834907471879No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCT181400.054.8892253
TACTCTA206900.049.2019044
TCTAGTT208700.048.282967
TGTACTC216050.046.375362
TTGTACT217250.045.9219781
GCCTAAC91650.043.969761
TAGTTGT234050.042.527149
ACTCTAG258650.039.5222135
CTCTAGT277550.036.6717076
CTAGTTG286550.034.5078058
CCTAACT136900.031.8506972
GGGGTTC193550.030.8124732
CGGGGTT343000.030.7584971
CGGGTTT227050.029.7409651
TGTCTTA94700.029.402225145
GGGTTCT223850.028.5412253
TAACTGC149200.027.0197474
CTAACTG153600.026.392133
GTTACTA92300.025.9278243
GAGTCTA51550.025.755522
Produced by FastQC (version 0.11.5)