FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n02_MT_30786.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_30786.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22565638
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13715206.077913684514482No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA447760.19842558849876082No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT407380.18053112435819452No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA347670.15407053857728287No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG334400.1481899160130106No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA304780.13506376376329357No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA303000.13427495380365492No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA252990.11211293915111109No Hit
GCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCC244320.10827081423534313No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCT189450.052.0933073
TACTCTA212750.047.1301544
TCTAGTT214650.046.4042557
GCCTAAC90300.046.147611
TTGTACT217750.045.175841
TGTACTC222650.044.473152
TAGTTGT229700.042.8901529
ACTCTAG260150.038.8192025
CTCTAGT281000.036.0662426
CTAGTTG281800.034.523018
CCTAACT137300.032.8102072
CGGGTTT215500.028.7360361
TGTCTTA94950.028.71561145
TAACTGC146200.028.1674294
CTAACTG153650.026.9477183
GTTACTA92350.026.7753983
CGGGGTT304700.026.4707781
GAGTCTA48300.025.3820952
GGGGTTC176800.024.7002942
GGGTTCT200400.023.8454863
Produced by FastQC (version 0.11.5)