FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n02_MT_30785.TR2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_30785.TR2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23160734
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAAGTGAAACATCTCAGTACCCGTAGGAGAAGGAAACAATATGTGAT662070.2858588160461581No Hit
GCGGGGAAGTGAAACATCTCAGTACCCGTAGGAGAAGGAAACAATATGTG520890.22490219869542993No Hit
CGGGGAAGTGAAACATCTCAGTACCCGTAGGAGAAGGAAACAATATGTGA346380.1495548457143025No Hit
CGGCGAATTAGTACCAGTCACCTCCACGCCTTACAACGCTTCCAGATCTA291590.12589842791683545No Hit
GGCCAATCAAACTCCGTGATAGCTGGTTCTCCCCGAAATGCATTTAGGTG274670.1185929599640495No Hit
GTGGAAGGCTGCGTTATGCCTCGGGGAGTTGTCAATCGAGCGTTGATCCG244630.10562273199113638No Hit
CGCGGGGAAGTGAAACATCTCAGTACCCGTAGGAGAAGGAAACAATATGT244430.10553637894204906No Hit
GTGAAAAGTACCCCGGGAGGGGAGTGAAATAGTTCCTGAAACCATGTGCT242160.10455627183490818No Hit
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATGCAA233910.10099420856005685No Hit
CGGCGAGCGAAAGCGAATGGTGGCTAAACCGTATGTGTGTGATACTTGGT233410.1007783259373386No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGTT40850.063.371765145
AGTGTAT49100.049.917732145
GGGAAAA111950.044.304886145
TGTTAAT60350.044.21709145
AGATTAT109800.038.370144145
GCGAATT138200.038.1527373
ATTAGTA160500.037.714737
GAATTAG143400.037.413565
AATTAGT147700.037.154976
TGTTAAA79000.036.532185145
GGCGAAT161000.032.8548852
CGTGTAT90950.029.65923145
CGGCGAA178400.028.1248591
AAGAGTT85550.025.852316145
TTTGATC166350.024.208532
CTGGCTC183400.024.0330819
GGTTCGC184650.023.7183654
GTTCGCG195650.022.3451825
GGGTTCG211650.021.7597963
ATGTAAT15400.020.71818145