FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n02_MT_30783.TR2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_30783.TR2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17043382
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC250470.14696026880111002No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG240170.14091686732128633No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTTA279600.049.844753145
AGCATTA118050.040.96664
GGGAAAA265800.039.965553145
CGTGTAT278150.038.29534145
GCATTAG127800.037.723655
CGTGTAA94200.033.484325145
CATTAGA158600.030.3948946
CCGTGTT188050.029.767828145
TCGTGTT170500.028.791712145
CAGCATT205450.025.168393
GGATATC86100.024.4382932
AAGAGTT216600.024.203772145
GATATCT93500.023.1916483
CCGTATA84600.022.19896145
GTAGATA346050.021.121506145
ATATCTT102600.020.9177234
AGAGCGG95250.020.630392145
TATCTTG92800.020.6242835
TAGAGGT240300.020.6041859
GAGCCGG146650.020.470171145
Produced by FastQC (version 0.11.5)