FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n02_MT_30783.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n02_MT_30783.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16921706
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC250280.14790470889873633No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG222270.1313520043428245No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTTA276750.048.522926145
AGCATTA114550.042.659284
GGGAAAA260900.039.270073145
GCATTAG125350.039.0967145
CGTGTAT276500.036.55622145
CGTGTAA88200.034.528233145
CATTAGA151150.032.4695746
TCGTGTT161500.030.62002145
CCGTGTT186500.028.265013145
CAGCATT196700.026.3592193
CCGTATA85650.025.905245145
GGATATC87500.024.3755152
AAGAGTT207950.022.455387145
GATATCT97250.022.2952563
TCGGAAA109450.021.862095145
TAGAGGT236650.020.9221559
GTAGATA346150.020.63315145
AGAGCGG96750.020.609875145
TATCTTG94450.020.2637525
GAGCCGG149200.020.02267145
Produced by FastQC (version 0.11.5)