FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41214524
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19780974.799514365372751No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT521880.1266252644334798No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT518400.1257809018975932No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTCT211000.023.2054633
CGGGTTT182300.022.2532541
GGGGTTC201200.021.3510682
CGGGGTT288850.019.7226661
CTTTTTT634250.017.3005391
GTTCTTT349200.015.5151325
CCCCTGT94900.015.1414441
CAGTCTA93800.014.37977125-29
GGGTTTT331950.014.2724312
CGTCTTG140100.013.560256145
GATCCTG123950.013.32344155
GGTTCTT483950.012.1828784
GCTGTGT99700.012.010391
TGCCGTG167550.011.338655145
TGATCCT142750.010.75686554
CTGTGTT204000.010.6888072
CGAGGGA127800.010.6544458
GAAACTA214350.010.6441994
CGGGAAC250150.010.5123312
TCCTGGC156050.010.3500467
Produced by FastQC (version 0.11.5)