FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n01_MT_8230.TR2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_8230.TR2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25777305
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT428960.1664099485962555TruSeq Adapter, Index 27 (97% over 39bp)
CTGAAACAAACATGCTAGTAAACTAGCGCCACTTGCGTTCAAAGATCTGA280640.10887096226700192No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATTAA230250.041.496723145
CGTCTTG152450.032.66836145
CTCGATG507600.027.163574145
AAACATG208900.024.1831198
ATCTAGG102950.024.009456
AACATGC206300.023.8555019
CAAACAT216300.023.8248987
CTGAACA210500.023.349396145
GCCAGTA124850.022.5266884
CATTACA139750.022.098143145
TCTAGGT125150.021.1387847
AGTAGTC127400.020.7654517
GAAACAA266950.020.0392133
ACAAACA249600.019.6025876
AACAAAC271100.019.5720255
CTGGCTC147800.018.782029
TATCTAG146900.018.7506375
TGCCGTG246650.018.487047145
ACACGTA100900.017.88983145
TGGATAT157450.017.541471
Produced by FastQC (version 0.11.5)