FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n01_MT_8230.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_8230.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25666380
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT427650.166618744053505TruSeq Adapter, Index 27 (97% over 39bp)
CTGAAACAAACATGCTAGTAAACTAGCGCCACTTGCGTTCAAAGATCTGA280000.10909212752246325No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATTAA219900.041.80085145
CGTCTTG153300.033.432457145
CTCGATG507750.028.554274145
ATCTAGG105550.025.8904446
CAAACAT212400.025.2180087
AAACATG216500.024.5395688
AACATGC207850.024.410069
GCCAGTA120250.023.8104934
CTGAACA210400.022.085302145
TCTAGGT124300.021.6916527
AGTAGTC124950.021.5788147
GAAACAA269900.021.243693
ACAAACA246800.021.1761256
AACAAAC267150.020.702645
CATTACA136650.020.689312145
TATCTAG150950.020.1204325
CTGGCTC144550.019.2545769
TGCCGTG244150.019.180807145
TGGATAT155650.019.1410241
GTCACAA160300.018.812689145
Produced by FastQC (version 0.11.5)