FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n01_MT_7920.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_7920.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27350587
Sequences flagged as poor quality0
Sequence length151
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT988900.36156445198050047No Hit
GGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGA929730.3399305470116601No Hit
GGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAG886650.3241795139533934No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA782680.2861657045971262No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT673670.24630915599727346No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG651880.23834223375169242No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG644020.2354684380265769No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC580350.2121892301616781No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG567040.2073227898180028No Hit
GGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGAC566130.20699007301013322No Hit
CGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGG554110.20259528616332806No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC551430.20161541688300874No Hit
CCTCCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTAC509180.18616785080334838No Hit
TGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCA506270.18510388826389723No Hit
GGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGAT490350.17928317224050805No Hit
CGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGA474660.17354654947625073No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC468270.17121021936384767No Hit
CCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCG463700.16953932286718382No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG453740.16589771912390766No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGA448980.16415735428274356No Hit
TCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGC445160.16276067493542276No Hit
CCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC444610.16255958235923784No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA437170.15983934823775447No Hit
CCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTG426080.15578459065613473No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT420370.1536968840924694No Hit
CCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGC410670.15015034229429883No Hit
GCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGC389900.14255635537182437No Hit
GGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAA388490.14204082713105937No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA387870.14181414095426909No Hit
CATGAATGGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAA368560.1347539634158492No Hit
CCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTC363620.13294778645884273No Hit
GCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAA360230.131708324943812No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA356200.13023486479467516No Hit
TGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACA345190.1262093570423187No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC344680.1260228893807654No Hit
CCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAGT333630.12198275671377729No Hit
CCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCG333310.12186575739672424No Hit
GTCAGGACCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGC330340.12077985748532563No Hit
CGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGC327400.11970492625990074No Hit
GAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGC327360.11969030134526912No Hit
GCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCCCG325350.11895539938502964No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG306450.11204512722158394No Hit
CGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGC301850.11036326203894638No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC295190.10792821375277979No Hit
GCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAG289930.10600503747872028No Hit
AAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGC285690.1044547965277674No Hit
TGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCC285020.10420982920768757No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA281210.10281680608902471No Hit
CTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCC280200.10244752699457602No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACTA238200.049.9074634
AAGAAAC243200.049.179642
AGAAACT247350.048.2078063
TCCCTGA205350.043.5595973
CTCGGCT89150.041.0629461
CTAACCA301600.039.4853028
TCAGATT59100.039.0035932
GCGATAT24050.038.281326145
CTCCCTG230900.038.143222
TTGAACG62000.037.6437957
AAACTAA326150.037.316145
GATTGAA63050.037.2497445
AACTAAC335900.036.31936
TACTTCC43350.035.4495055
ATAGATG100950.035.04385145
TAAGTTA30350.034.39292
TAACCAG384150.034.0758069
CTGTAAC119900.033.977951
TTACTTC45600.033.7003484
ATCGAAG72400.033.540516
Produced by FastQC (version 0.11.5)