FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n01_MT_7784.TR3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_7784.TR3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24481343
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT376270.15369663339139522TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGATG297400.038.2206145
CGTCTTG156500.025.19863145
GTCACAC177950.021.916775145
GCGATAT105100.021.589073145
ATAGATA278150.019.96375145
CTGAACA221250.019.134714145
ACACGTA98850.018.187265145
TAGTTGT116200.015.8434559
TCAGATT214100.013.8473912
CAGATTG193450.013.6392763
TCTGAAA166050.013.228059145
AGATTGA222150.013.0844874
GTTTGAT323900.012.9137621
TGCCGTG253400.012.873549145
GTACTCT150600.012.8031053
GATCCTG188950.012.6981565
CACAGCC215550.012.510864145
TTTGATC290800.012.4384852
ATGTAAC119500.012.3136756
TGTACTC153250.012.2979912
Produced by FastQC (version 0.11.5)