FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n01_MT_7784.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_7784.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24294703
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT374500.15414882824457662TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGATG287500.036.86524145
CGTCTTG155050.025.01437145
GTCACAC168750.021.394053145
ATAGATA280400.020.864264145
GCGATAT108050.020.597815145
CTGAACA229500.019.42674145
ACACGTA95050.017.160816145
TAGTTGT110900.016.535119
TCTGAAA163850.013.892831145
GTACTCT145950.013.4591413
CAGATTG193600.013.2915333
TCAGATT217800.013.2791362
GATTGAA228950.012.7906575
TGTACTC153600.012.7416982
CTGGCTC156450.012.74017059
TGCCGTG249550.012.724012145
AGATTGA228100.012.4887624
TTTGATC286150.012.4631022
GATCCTG193500.012.2120555
CGTCTTA92600.012.05639145
Produced by FastQC (version 0.11.5)