FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n01_MT_7431.TR4_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_7431.TR4_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26398928
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT494070.18715532691327466TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGATG440950.038.503254145
CGTCTTG177300.034.713535145
GCGATAT125800.025.182604145
CTGAACA256000.022.880802145
GTCACAC233700.022.830687145
TGCCGTG243900.022.618965145
ATAGATA415800.020.90425145
CACAGCC195100.019.879084145
CTGGCTC126650.019.3436769
CTTCTGG213700.019.302235145
GATCCTG185350.015.2523995
ACACGTA124500.015.022779145
TCTGAAA211650.014.419948145
CGTCTTA129350.013.114426145
GCGATGT140450.012.9532729
GTTACGA120300.012.8345344
TGATCCT233450.012.5134584
TCCTGGC209000.012.24217
TCCAGTA247300.012.194643145
AGCATTA129000.011.9689484
Produced by FastQC (version 0.11.5)