FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n01_MT_7431.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_7431.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26246627
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT492180.18752123844332455TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGATG443600.040.855263145
CGTCTTG174450.032.70418145
CTGAACA251050.025.757526145
GCGATAT129950.023.262657145
TGCCGTG241700.022.494904145
GTCACAC232000.022.123041145
ATAGATA410400.021.44425145
CACAGCC191700.019.626587145
CTGGCTC127550.018.9232039
CTTCTGG208450.017.458275145
GATCCTG182100.014.8481655
TCTGAAA207350.014.753937145
ACACGTA129750.014.1355505145
GTTACGA124150.013.1957964
CGTCTTA130400.013.120003145
TCCTGGC196950.012.3287687
GCGATGT142450.012.3135649
GAGCACC227350.012.180579145
TGATCCT238950.011.8009374
CCTGGCT195750.011.7748728
Produced by FastQC (version 0.11.5)