FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n01_MT_6852.TR3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_6852.TR3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25074975
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT298580.11907489439171924TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGATG207900.028.663595145
ATAGATA178400.018.692884145
TAGTTGT113900.016.735879
GTCACAC155300.016.665182145
GTTACGA87550.016.475954
CACTATA81000.016.375049
CTGAACA230000.015.980613145
ATACGAC82850.015.6618671
TTACGAC98400.014.953935
ACACGTA96100.013.427957145
GTACTCT148250.013.1525853
ACTCACT117550.012.8878056
CTTGTTA119250.012.8872571
TGTACTC155750.012.8450642
GTCTAGG66400.012.5548861
TCTGAAA143000.012.420622145
TTGTACT151250.012.0778081
TACGACT133550.011.7237126
TCTAGTT188350.011.4674527
TACTCTA183450.010.9449964
Produced by FastQC (version 0.11.5)