FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n01_MT_6852.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_6852.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24929301
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT297220.11922516399477065TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGATG200950.027.41757145
ATAGATA176850.018.651325145
GTCACAC148750.016.765049145
CACTATA84500.015.7839
TAGTTGT114500.015.3825829
CTGAACA228950.015.325268145
ATACGAC81550.014.5781
GTTACGA92600.013.0725424
GTACTCT148200.012.8635883
ACTCACT117800.012.7988996
ACACGTA89300.012.420622145
TCTGAAA145100.012.040734145
TTACGAC101700.011.9741015
GTCTAGG67200.011.9737941
TGTACTC156850.011.9231392
CTTGTTA120450.011.7957991
TTGTACT153700.011.5078461
TCTAGTT183350.011.4642477
GACTCAC140350.011.4139095
ATAATAC80750.011.4002873
Produced by FastQC (version 0.11.5)