FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n01_MT_6751.TR4_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_6751.TR4_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23697111
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT328470.13861183331588395No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT324650.13699982246781053No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATAC77200.038.4955943
AGTCACA83500.038.200363145
GCCAGTA109500.034.885254
TAGCATA112550.032.842089
GCTCAGA124700.032.2024355
GTAGCAT116300.032.094718
CACTATA104100.031.3305329
AGTCTAA112500.030.4756573
CGTATGG84100.030.169863145
GAGCATT103600.029.6627948
GAGTCTA123750.027.8223482
TACGACT123500.027.2330572
TCTAACA129550.026.6886355
TAATACG106900.026.5121174
TTGTACT76600.025.0777761
CGCTCAG132150.025.0116884
TGTACTC77650.024.7371582
AACGTAT215700.024.699186
ACTCACT132150.024.6825896
ATACGAC126400.024.5453151
Produced by FastQC (version 0.11.5)