FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n01_MT_6751.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_6751.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23588025
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT327480.1388331579265326No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT323720.13723912875283115No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACA83000.043.147057145
ATAATAC77850.040.969223
GCCAGTA105900.033.813854
GCTCAGA123150.031.9027775
GTAGCAT111850.030.9755298
CGTATGG83450.030.839285145
TAGCATA112700.030.7419079
CACTATA103900.030.6947389
GAGCATT99900.030.1099078
AGTCTAA111700.029.786753
TAATACG105650.028.6108154
TACGACT130000.027.2641747
TCTAACA125100.026.6541085
GAGTCTA125850.026.3226382
AATAATA117550.026.2695942
GTTACGA51200.026.1918054
TCACAGC89600.025.564823
GTCACAG90000.025.3708172
AACGTAT210050.025.1230496
CGAAATA127550.024.950909145
Produced by FastQC (version 0.11.5)