FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n01_MT_5867.TR1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_5867.TR1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28913034
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATTAA222700.040.591755145
CGTCTTG151200.030.684551145
CGATCTA111000.023.9657144
CTCGATG644800.022.282824145
CTATTAG159600.020.118158
CTGGCTC211250.020.0370319
GTCACAA150600.019.543064145
GATCCTG242200.017.6573355
CTGAACA290550.017.465006145
ATCTATT181000.016.7395976
CTCGATA357250.016.273977145
CATTACA195750.016.035324145
TGCCGTG266000.015.425043145
TCTATTA182950.015.37151057
TCTGAAA161600.015.296942145
TGATCCT282350.014.4276564
GAGCACC202750.013.944256145
TATTAGT250650.013.4462919
CCTGGCT303450.013.2802098
TCCTGGC306100.013.2362737
Produced by FastQC (version 0.11.5)