FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n01_MT_5867.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_5867.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28793551
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATTAA220150.042.148827145
CGTCTTG152300.030.605907145
CGATCTA112150.022.6877144
CTCGATG636300.021.817303145
CTATTAG160250.019.4044888
GTCACAA155400.018.846231145
CTGGCTC210850.018.7011459
GATCCTG238550.016.8350055
CTGAACA289900.016.15394145
TGCCGTG265900.015.921674145
CTCGATA361400.015.665963145
ATCTATT182950.015.53236
TCTGAAA159550.015.039222145
CATTACA197600.014.4912145
TCTATTA184750.014.3202737
TGATCCT277150.014.2549114
TATTAGT247100.013.5816079
TACTCGC121700.013.521643145
GAGCACC206100.013.225255145
TCCTGGC307300.012.9259037
Produced by FastQC (version 0.11.5)