FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n01_MT_4340.TR1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_4340.TR1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26153841
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACGAGCAATTAGTATGGGTTAGCTTCACGCGTTACCGCGCTTCCACA339110.12965973143294707No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCA128950.045.4761054
TCACGAG125500.045.109112
CAATTAG178600.037.4163639
CGAGCAA180450.034.7066735
GCTCAGA99350.033.197065
ACATTAA88900.032.3735145
TGATCCT170350.032.0837674
GCAATTA215100.031.5726688
AGCAATT205000.031.0775767
GATCCTG196500.028.0723135
GAGCAAT252300.025.684816
CAGATTG152600.024.7954543
CGGAGTA93700.024.2910424
TCCTGGC234100.022.8180317
TTTGATC277700.022.2131732
CTGGCTC254250.021.294719
CCTGGCT259250.020.8560548
GGTTAGA197750.020.6737254
GGGCACA38350.020.2260481
AGATTGA257900.020.124164
Produced by FastQC (version 0.11.5)