FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n01_MT_4340.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_4340.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26053110
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACGAGCAATTAGTATGGGTTAGCTTCACGCGTTACCGCGCTTCCACA338400.129888523865289No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAG122100.043.9320642
ACGAGCA128950.042.4414634
GCTCAGA102600.036.1025855
CAATTAG180000.034.7107249
ACATTAA90100.034.597927145
CGAGCAA177450.032.72065
TGATCCT171650.030.5745094
GCAATTA209250.030.3782718
AGCAATT199200.029.6185637
CGATCTA30900.027.2122384
GGGCACA38600.026.2923641
GATCCTG201750.026.1926085
CGGAGTA94700.024.1881054
GAGCAAT251550.024.090556
CAGATTG153250.022.656863
TCCTGGC230850.022.418027
TTTGATC282700.021.7438092
GGTTAGA199750.020.9388964
CTGGCTC252150.020.5530349
CCTGGCT259000.020.0374378
Produced by FastQC (version 0.11.5)