FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n01_MT_4329.TR3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_4329.TR3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28390469
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGTCCTACTCTCCCACAACCTATCGGTTGCAGTACCATCGGCGCTG290040.10216104566641714No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCTA194900.033.769575
CTATTAG135350.033.307938
AGTCACA106350.031.765003145
TCGATCT88400.030.0109923
CGATCTA90650.029.7458694
GATCCTG148100.028.974655
CGTATGG108100.026.019953145
GTGTCCT265700.025.0166594
ATCTATT131100.023.3877896
CGAAATG296450.022.424274145
TATTAGT208050.022.4005589
GAAACTA115000.022.3759784
TTTGATC253300.022.3497142
CTGGCTC205700.022.0927019
CGGTGTC280300.021.5933252
TCCTACT321350.020.8405557
CTCGATC134500.020.479412
AAATCTG149950.020.256672145
CAATTAG91900.020.0324369
CTACTCT345050.019.5981569
Produced by FastQC (version 0.11.5)