FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n01_MT_4329.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_4329.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28248287
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGTCCTACTCTCCCACAACCTATCGGTTGCAGTACCATCGGCGCTG289170.10236726920821783No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCTA194700.034.8101465
CGATCTA88550.032.5802734
TCGATCT87750.031.5556013
CTATTAG142200.031.4489258
AGTCACA101300.030.700737145
GATCCTG145600.028.2779035
GTGTCCT269250.025.5488154
ATCTATT127800.025.410166
CGTATGG103300.024.63246145
GAAACTA115050.023.6898024
CGGTGTC283700.022.2801512
TATTAGT211150.022.1405749
TCCTACT326600.021.5488117
CTCGATC132050.021.4085462
AAATCTG144350.020.992321145
CTGGCTC208150.020.8230829
CGAAATG296250.020.702032145
TTTGATC254650.020.6658782
CTACTCT348150.020.3190619
CAATTAG94700.019.8230299
Produced by FastQC (version 0.11.5)