FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n01_MT_30981.TR3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_30981.TR3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24281587
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT408450.1682138815720735TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGATG330900.039.32584145
GCGATAT82200.033.425484145
CGTCTTG174650.026.109127145
CTGAACA214250.024.599363145
GTCACAC200500.022.815254145
TGCCGTG184550.022.665854145
ATAGATA313600.022.238697145
ACACGTA94650.018.918499145
TCTGAAA160800.016.68115145
CACAGCC235350.015.740446145
CTTCTGG223400.014.278409145
AGATTGA223750.013.9630364
CGTCTTA99350.013.937209145
GATCCTG147750.013.7861965
GATTGAA198050.013.46915
CAGATTG209250.013.3370833
GAGCACC190950.012.832385145
AACGTAT227950.012.5927675
TCAGATT204800.012.5650952
AGTTAGA133150.012.3580594
Produced by FastQC (version 0.11.5)