FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n01_MT_30981.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_30981.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24123660
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT406810.16863527342036821TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGATG321100.038.244743145
GCGATAT83500.033.598747145
CGTCTTG176850.027.423298145
GTCACAC194950.024.691273145
CTGAACA213500.024.17579145
ATAGATA304850.024.089172145
TGCCGTG183600.022.466644145
ACACGTA95300.016.506897145
TCTGAAA154100.016.323957145
CGTCTTA98650.015.652408145
AGATTGA224200.015.1637334
CTTCTGG216450.014.836953145
GATCCTG153250.014.5213435
GATTGAA202300.014.3686985
CAGATTG211450.014.1240233
CACAGCC228300.014.035083145
AGTCTAA101100.013.8380565
TCAGATT204350.013.4087092
CTCGGCT110950.013.0675751
GAGCACC190750.012.99751145
Produced by FastQC (version 0.11.5)