FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n01_MT_30976.TR2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_30976.TR2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29629900
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA517750.17473903050634665No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT461580.155781828490815No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT458300.15467483859209785No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT381780.128849574247635No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG379750.12816445549934358No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT379440.128059831454038No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC347760.11736792901764773No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT339340.11452620494837985No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC336990.11373308718557944No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA331540.11189372896972315No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC325610.10989237223210338No Hit
CATGAATGGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAA311340.105076291178843No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG297300.10033783441726093No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA296910.10020621061832811No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATATA26150.037.148376145
TAGCATA117150.035.6991738
GCCAGTA117550.035.2729073
GCTCAGA119200.034.9671485
GTAGCAT122400.033.9903077
CTCGGCT117150.032.9206161
CTTGTTA60800.032.0735741
CGCTCAG114800.031.3190354
TGCCAGT130300.031.2654672
GTTACGA69950.030.9850394
TACTTCC155600.030.3277365
TTACTTC158550.029.7634564
CCTGCCA124800.029.3923681
TATGATG112300.029.24322145
AGTCTAA185450.028.7686083
CCAGTAG146650.028.3231244
GAGTCTA189650.028.2082752
TCTAACA193850.027.8211925
ACTTCCT174000.027.2873066
CAGTAGC154750.027.0748315
Produced by FastQC (version 0.11.5)