FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n01_MT_30893.TR3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_30893.TR3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27068461
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT300700.11108869469897088TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATTAA167150.050.440403145
CGTCTTG120800.032.946598145
CTCGATG450900.024.45424145
CATTACA104750.021.108192145
CTGAACA216100.020.362877145
CAATTAG102900.019.7927239
TAGTCAT91000.018.7172079
CTCGATA274050.018.120325145
CTGGCTC131050.018.0299769
CGATCTA76400.017.2677884
AGTAGTC94050.016.9542457
CTATTAG104650.016.4143498
TATCTTG95600.016.3019395
ACGAGCA104500.016.300854
TGCCGTG207150.016.098228145
GCCAGTA108700.016.0044364
GTAGTCA106800.015.6088598
GCACACC181400.015.54596145
TACTCGC89100.014.808085145
TACGACT116550.014.2423586
Produced by FastQC (version 0.11.5)