FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n01_MT_30893.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_30893.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26958089
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT299820.11121708219006177TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATTAA167700.048.19887145
CGTCTTG118300.033.029278145
CTCGATG456350.025.94079145
CATTACA105850.024.58661145
CAATTAG102750.021.4443429
CTGAACA217450.020.369335145
CTCGATA267300.019.0114145
TAGTCAT92450.018.8159129
AGTAGTC91350.018.5664237
CTGGCTC126700.018.1344179
CTATTAG99200.018.1201158
ACGAGCA102750.018.0603034
CGATCTA74600.017.8791084
GTAGTCA105200.016.7421598
CGAGCAA122400.016.3453625
GCCAGTA108300.015.9299854
TGCCGTG211650.015.652835145
GCACACC179700.015.652321145
CAGTAGT114700.014.661946
CCTGCCA124150.014.42252351
Produced by FastQC (version 0.11.5)