FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n01_MT_30893.TR2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_30893.TR2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27172839
Sequences flagged as poor quality0
Sequence length151
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTG532620.19601190733143492No Hit
CCTCCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTAC469390.1727423476067407No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT408910.1504848278827251No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC407010.1497856002458926No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG365210.1344025922355776No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC339470.12492989782922571No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT333270.12264820764587755No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA329570.12128655382678269No Hit
GGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAG315860.11624107440521764No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT315230.11600922524142583No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG300600.11062517243781557No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT300250.11049636734682011No Hit
CCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGC286750.10552817097985234No Hit
CGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGG286270.10535152399791571No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC285310.10499823003404245No Hit
CGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGA281330.1035335321421512No Hit
GGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGAT274140.10088750755855874No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTAA193950.044.2509355
GAGTCTA202000.042.092744
GTAGCAT82700.041.5434158
TCTAACA219700.039.094687
TAGCATA87450.039.038269
GCCAGTA91200.037.3562744
GTCTAAC235250.036.32826
CGCTAGG27350.034.4545484
GAAACTA261800.034.000814
CCAGTAG101850.033.5924385
AGGAGTC262000.032.425812
TGCCAGT104300.032.1778643
TACTTCC142850.031.4609625
AAGAAAC293850.030.4653242
CTTTTAC147900.030.2909431
CAATTAG48500.030.1883229
CTAACCA293200.030.0112518
CCTGCCA113050.029.5612551
AGAAACT305150.029.3368933
TCCCTGA304600.028.985313
Produced by FastQC (version 0.11.5)