FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n01_MT_30852.TR3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_30852.TR3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27017595
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACA123800.031.79723145
GATCCTG140450.028.7987965
CGTATGG120350.028.070486145
GAGCAAT127050.026.7583456
CAATTAG130500.026.6597239
ACGAGCA121300.024.7998584
TCACGAG120900.024.7021752
CACTATA58050.022.7244839
AAATCTG155750.022.714432145
CTATTAG98800.021.4215458
TTTGATC213150.021.3909262
CTGGCTC187350.021.2007759
CGAAATA182600.020.883104145
TACGACT98650.020.7212662
CGAAATG343200.020.595268145
AGCAATT159400.020.4620487
TCCTGGC192050.020.3422667
TGATCCT200800.019.9989834
CCTGGCT200500.019.6295498
CGAGCAA168000.019.4162395
Produced by FastQC (version 0.11.5)