FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n01_MT_30852.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_30852.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26882147
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTG132150.031.1007445
AGTCACA124000.029.991503145
CAATTAG128850.024.6382039
GAGCAAT125950.024.4018386
CGTATGG115400.024.311272145
TTTGATC214150.022.4415442
TCACGAG122200.022.3035452
ACGAGCA124900.021.7632474
TACGACT99150.021.7130582
CACTATA56550.021.5325769
CGAAATA173300.021.334091145
CTGGCTC187900.020.7141369
TCCTGGC195050.020.5493687
CTATTAG101050.020.5139378
TGATCCT201300.020.4891224
AAATCTG152650.020.088432145
CGAAATG339450.019.904102145
CCTGGCT199550.019.7953918
AGCAATT152200.019.2867777
GCTCAGA131600.019.1129825
Produced by FastQC (version 0.11.5)