FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n01_MT_30852.TR1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_30852.TR1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29397764
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT302570.10292279371995774TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATTAA183500.039.822056145
CGATCTA130350.027.248294
CTATTAG161700.025.101168
CGTCTTG161650.021.570917145
ATCTATT200400.018.3385586
CTGAACA247550.017.717062145
TCTATTA205650.017.5515887
TATTAGT255150.017.2428469
GTCACAA144350.017.225689145
GATCCTG224850.016.699055
CTCGATG673400.016.180244145
CTCGATA284550.015.973797145
CTGGCTC229000.015.0339789
GCGTTTT87500.014.581557145
TGATCCT261200.014.1530894
TCTGAAA160100.014.0821295145
CATTACA169600.013.891744145
GATCTAT289850.013.6044365
CGTCTTA79900.013.428089145
CGTACGG99500.012.7487953
Produced by FastQC (version 0.11.5)