FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n01_MT_30852.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_30852.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29287209
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT301420.10291864957155869TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATTAA181400.039.084034145
CTATTAG150800.024.368968
CGATCTA128500.024.0872784
CGTCTTG158700.021.103909145
GATCCTG230250.016.7484635
CTGAACA256000.016.70744145
TATTAGT242700.016.6645959
ATCTATT203300.016.6510836
CTCGATG677300.016.579374145
GTCACAA141100.016.38934145
TCTATTA204300.016.1780747
CTGGCTC228000.015.736239
CTCGATA294250.014.510977145
TGATCCT258250.014.1466354
CATTACA164400.014.066522145
TCTGAAA163100.013.156354145
GCGTTTT86750.013.119823145
CGTCTTA79900.012.520743145
CGGAGTA119300.012.5166984
TAGTTGT144050.011.8748769
Produced by FastQC (version 0.11.5)