FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n01_MT_30844.TR4_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_30844.TR4_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25325628
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1057980.4177507463980755No Hit
GGAATATTAACCTGTTTCCCATCGACTACGCCTTTCGGCCTCGCCTTAGG361950.14291846978088757No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT352600.13922655738290082No Hit
CTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACG348060.13743390687093723No Hit
GCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCC339380.13400654862339445No Hit
CAGCTATCTCCCGGTTTGATTGGCCTTTCACCCCCAGCCACAAGTCATCC271280.10711679094394028No Hit
CCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCAC263240.10394214113861264No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC261350.10319586152019607No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATTA81650.076.253177
ATCGATT82300.076.097576
GATCGAT84950.073.7237245
CGATTAG84750.073.207398
TCGATCG100450.062.41993
CGATCGA101800.061.734544
GATCCAA80100.052.396585
GCGACTA43650.051.147582
CCTAACT114600.049.0835232
TTGAACG67050.046.1583187
GATCCAG166150.044.5869185
GATTGAA73150.043.799065
ATCCAAC98500.043.1975146
ATCCAGC174800.042.6708036
TCAGATT73650.041.435062
GGAAGCA142250.040.4599273
CAGATTG77700.040.0214843
CGCGACT22950.039.7989271
GCCTAAC144800.039.449471
TAACTGC143300.039.050634