FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n01_MT_30844.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_30844.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25181380
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA861400.3420781545729424No Hit
GGAATATTAACCTGTTTCCCATCGACTACGCCTTTCGGCCTCGCCTTAGG360660.14322487488771465No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT351510.139591237652583No Hit
CTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACG347120.1378478860173668No Hit
GCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCC338370.1343730963116398No Hit
CAGCTATCTCCCGGTTTGATTGGCCTTTCACCCCCAGCCACAAGTCATCC270430.10739284344225773No Hit
CCTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCAC262360.10418809453651866No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC260660.10351299253654883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAT83800.078.023095
ATCGATT84350.077.342476
TCGATTA85450.076.1720357
CGATTAG85950.075.054278
TCGATCG101300.064.472723
CGATCGA102850.063.5715564
GCGACTA41650.053.951972
CCTAACT108400.050.286242
GATCCAA82700.048.12025
GATCCAG158200.043.0707055
TTGAACG67550.042.813317
ATCCAAC98050.041.695786
ATCCAGC165100.041.2706576
TAACTGC134650.041.0751154
ATTCGCC28650.039.220135145
GGAAGCA144800.039.1970863
TCATTAG111700.039.196233
GCCTAAC141350.039.129781
GATTGAA75150.039.064895
TCAGATT73400.037.823642