FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n01_MT_30830.TR1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_30830.TR1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24163615
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7038552.9128712736070326No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1304930.5400392284018761No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1132970.46887437993032083No Hit
GTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT421600.1744772046732246No Hit
CGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT365040.1510701109912569No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT361580.14963820603829353TruSeq Adapter, Index 19 (97% over 38bp)
CTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACG303090.12543239080741853No Hit
CCGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302810.12531651410602263No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298210.12341282544023319No Hit
GCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCC295460.12227475069438079No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAAC110850.045.77841
CGTTTTT60900.044.638771
CTTTTTT554300.040.647551
CGATTAG81800.039.6963968
ATCGATT86400.038.8446586
TAGTTGT135350.038.7174159
TTGTACT144150.035.9072231
TGTACTC146200.035.7481652
TACTCTA160800.033.4038284
CCTAACT162450.033.1539272
TCTAGTT167850.032.3434837
ACACGTA29700.031.975582145
CTCTAGT170050.031.5868026
ACTCTAG172350.031.4176315
TCGATCG109800.029.7740763
GTACTCT176100.029.5137143
GATCCAG169250.029.080725
GATCCAA107700.028.2008635
CGATCGA120250.028.090854
TAACTGC182100.027.4664944