FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n01_MT_30830.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_30830.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23873795
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5635192.3604081378766972No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1300160.5445971199802964No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1127840.4724175607606583No Hit
GTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT419760.1758245808846059No Hit
CGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363550.15227993706069773No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT360280.15091023442230278TruSeq Adapter, Index 19 (97% over 38bp)
CTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACG301920.12646502158538264No Hit
CCGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301030.12609222790092653No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296900.12436229765732677No Hit
GCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCC294450.12333606785180151No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAAC107550.046.643151
CGTTTTT56150.044.670271
CTTTTTT539000.040.778631
CGATTAG80250.038.11588
ATCGATT85450.037.750916
TAGTTGT133250.037.370359
ACACGTA27050.037.251934145
TTGTACT140700.034.6747361
TGTACTC142600.034.567572
TACTCTA153750.033.5222434
CCTAACT164700.032.0858422
TCTAGTT164500.030.755817
ACTCTAG166750.030.256795
CTCTAGT168600.030.053786
GTACTCT169000.029.0817573
GCGACTA41000.028.9960922
GTTACTA58900.028.7990533
GATCCAA106200.028.1906455
TAACTGC179850.028.0125684
GATCCAG163550.027.657545
Produced by FastQC (version 0.11.5)