FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n01_MT_30786.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_30786.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22565638
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10293914.561763332372876No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2272301.0069735231948682No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1929330.8549857974323616No Hit
GTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT733210.32492323062170897No Hit
CGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT699030.3097763067899964No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT535390.23725896870276836No Hit
CCGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT512210.2269867131609574No Hit
CTGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT385590.17087484962756203No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338390.14995809114725672No Hit
CGGGGTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330980.1466743373265139No Hit
CCGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319540.14160468230501613No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT312220.13836081213391796TruSeq Adapter, Index 19 (97% over 38bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT304710.13503274314690328No Hit
GCTGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296630.13145207771213913No Hit
GGGGTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293710.1301580748570016No Hit
GGGTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277490.12297015488771024No Hit
GCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCC270510.11987695628193629No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268440.11895963234010933No Hit
GGTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260070.11525045292315689No Hit
CCGGGGTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259870.11516182259061322No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258750.11466549272836869No Hit
CCTGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246630.10929449457622248No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240410.10653809123411445No Hit
CGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231000.10236803408793493No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAAC92950.046.483041
CTTTTTT968850.040.659381
CGTTTTT104700.039.8816831
GTACTCT189500.036.876373
GCTGTGT132250.035.0818061
TCTAGTT200500.035.066977
TACTCTA204950.034.450174
CGGGTTT330650.032.557841
TTGTACT214650.032.354321
TAGTTGT216150.032.1591269
TGTACTC219700.031.7414152
CCTAACT147300.031.2501622
CGGGGTT391300.028.7516772
GTTACTA86900.028.445593
CTGTGTT306650.028.3674032
GAGTCTA44800.028.1548062
ACTCTAG257900.027.9392915
TAACTGC153550.027.9008874
GGGTTCT326050.027.279773
CCGGGGT375050.027.1378611
Produced by FastQC (version 0.11.5)