FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n01_MT_30785.TR2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_30785.TR2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23160734
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAAGTGAAACATCTCAGTACCCGTAGGAGAAGGAAACAATATGTGAT550490.237682449960351No Hit
GCGGGGAAGTGAAACATCTCAGTACCCGTAGGAGAAGGAAACAATATGTG328670.1419082832176217No Hit
GGCCAATCAAACTCCGTGATAGCTGGTTCTCCCCGAAATGCATTTAGGTG286620.12375255464701594No Hit
GTGAAAAGTACCCCGGGAGGGGAGTGAAATAGTTCCTGAAACCATGTGCT276010.11917152539293445No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTATCTCAGTCCCAATGT256180.1106096205759282No Hit
CTTTCGCTACGACTACCCCACACGGGTTAACCTCGCGACATGCCGCTGAC244180.10542843763068994No Hit
CGGCGAATTAGTACCAGTCACCTCCACGCCTTACAACGCTTCCAGATCTA238920.10315735243969384No Hit
GTGGAAGGCTGCGTTATGCCTCGGGGAGTTGTCAATCGAGCGTTGATCCG234660.10131803249413425No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGTA146750.032.0049867
GCGAATT129250.030.6202753
GAATTAG133000.030.2474235
AATTAGT138350.029.8636366
GGCGAAT152400.026.3020742
CTGAACA71250.023.60513145
TTTGATC167750.023.2903522
CTGGCTC183600.022.896879
CGGCGAA173700.022.035141
CAGGGGA41300.020.536081
ATGTAAT11600.019.373451145
TGATCCT215950.018.863844
GATCCTG224250.018.585855
GTCACTT34200.018.439124
GTTTGAT247050.018.3683931
GGTTCGC173100.018.3410434
GAAGTGA565700.017.7207874
GGAAGTG565550.017.712673
AATCGCC203500.017.6314416
TGGATGC179450.017.4496733
Produced by FastQC (version 0.11.5)