FastQCFastQC Report
Wed 20 Nov 2019
H7GKMBGXC_n01_MT_30783.TR2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_30783.TR2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17043382
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460200.27001683116649033No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT449520.2637504692437217TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATTAA127450.040.896446145
GGATATC82450.024.4406322
CTCGATG318500.023.011148145
CTGAACA140150.022.70743145
GATATCT95050.021.887033
CGTCTTG101100.021.439596145
TATCTTG93800.019.3967045
ATATCTT99650.018.9854224
ACACGTA67350.017.975271145
CATTACA97850.017.854708145
GTCTAGG56900.017.1991331
GCCAGTA81150.016.5249544
TAGGTTC62350.016.1597774
CGGATAT114450.015.8980191
GTTACGA94850.015.742984
TTACGAC98250.015.5670715
CTCGATA211100.015.556308145
AGCATTA93250.015.0802974
GACACTC37050.015.0647132
TCTTGGC115250.014.904897
Produced by FastQC (version 0.11.5)