FastQCFastQC Report
Thu 5 Sep 2019
H7GKMBGXC_n01_MT_30783.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKMBGXC_n01_MT_30783.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16921706
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT448020.2647605389196574TruSeq Adapter, Index 27 (97% over 39bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA171080.10110091736613318No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATTAA128650.042.03773145
GGATATC83250.027.1667842
CTGAACA135600.025.822466145
GATATCT96600.023.8625373
CTCGATG308150.023.737734145
CGTCTTG103500.023.18451145
TATCTTG91750.021.489725
ATATCTT99550.020.4612274
CGGATAT114950.018.793541
CATTACA95050.018.076174145
AGCATTA98900.017.3707544
GAGTCTA42550.017.0359764
ACACGTA64350.017.011345145
GTCTAGG54950.016.490871
GTTACGA87550.015.731284
TCTTGGC122350.015.5211597
GCCAGTA81450.015.4854834
TTACGAC93250.015.0029395
GCACACC159550.014.948904145
GTAGTCA81700.014.6382368
Produced by FastQC (version 0.11.5)