FastQCFastQC Report
Wed 25 Apr 2018
H7GKLBCX2_l01n01_833701tr2.351000000d254b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GKLBCX2_l01n01_833701tr2.351000000d254b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25884916
Sequences flagged as poor quality0
Sequence length101
%GC79

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGC4831281.8664460800259117No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC4138931.5989737034495304No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG3915391.5126145280904137No Hit
CGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCG3501531.352729906482988No Hit
CGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCA3286051.2694845136835675No Hit
GCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAA2450830.9468178301216044No Hit
GCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACC2381860.9201729686895641No Hit
CGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCC2176270.8407483338945353No Hit
GGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGG2106950.81396825857963No Hit
GGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGC2062720.7968810870392626No Hit
CGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGC2038510.7875281495987856No Hit
CTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTC2020640.7806245150650672No Hit
CCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGG1873020.7235951625263146No Hit
CGGGAACCGAAGAAGCGGGGCGCGCCGACCGGGGTCGCGCGCCCTCCCCC1741950.6729594950201886No Hit
GTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCG1709330.6603575611371504No Hit
TTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGT1708550.6600562273410506No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGG1688900.6524649336316177No Hit
GCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCC1623780.6273074249110949No Hit
GGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCC1495430.5777225624375215No Hit
GTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGG1384560.5348906675996167No Hit
GGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGT1377570.5321902531961086No Hit
CCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGG1245140.48102918317370624No Hit
CGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGAC1193550.4610986568393731No Hit
GGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCG1105240.4269822625655807No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC1047480.4046681086390236No Hit
CGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCC1022710.3950988289859623No Hit
CCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCC1006210.3887244602223163No Hit
GCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCG984830.38046482360614964No Hit
CGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAAC949610.36685844373611254No Hit
CCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCG938670.3626320440831255No Hit
CCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCG923350.3567135392674251No Hit
CGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGG909880.3515097364040123No Hit
GAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCG903030.34886340755365014No Hit
CAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGG868350.3354656433886052No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT860240.3323325445599283TruSeq Adapter, Index 6 (97% over 36bp)
GGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGC849200.3280675123689797No Hit
GGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCC846080.32686217718458116No Hit
GCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCC831950.3214033995706225No Hit
CTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC766280.29603341189131155No Hit
CCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGGG761670.2942524518912868No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG745570.2880326132794868No Hit
GTCCGCTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCG719290.27787998230320704No Hit
TCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCC702780.2715017502857649No Hit
CTCGACACAAGGGTTTGTCCGCGCGCGCGCGCGCGCGTGCGTGCGGGGGG687110.2654480315871993No Hit
CGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCG679920.26267035210776807No Hit
GGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGA601080.23221245917892877No Hit
CGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCTCCCAAAC600150.23185317657588692No Hit
CCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGT599910.2317604584847793No Hit
GGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCG597470.23081782455851896No Hit
CGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTC576560.22273976087077121No Hit
GCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCCC573670.22162328052368416No Hit
CCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGGGGGGAGGG570710.220479757400024No Hit
GAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGC568030.21944440538265605No Hit
CTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTG560930.21670149518739024No Hit
CGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGC558340.2157009124541876No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC556100.2148355436038502No Hit
GGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCG552130.21330183184677903No Hit
GCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGC544650.21041211800725954No Hit
CTCGTCCGCTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCC538390.2079937211308702No Hit
GGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGT535390.20683474499202545No Hit
GGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACCGGCG535050.2067033943629564No Hit
CGGGGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCT515110.19900006629343514No Hit
TGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGC510230.1971147984409144No Hit
CTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGC507330.19599445484003117No Hit
CGGCGCCGTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGC506010.19548450533893946No Hit
CCGCTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGA505790.1953995137554242No Hit
CGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGC505760.19538792399403573No Hit
CGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGC501320.19367263930854556No Hit
CGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGGGA490020.1893071625188971No Hit
TTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCC484090.18701625301778071No Hit
CGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGC482960.18657970533881585No Hit
CGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCCTC475350.1836397691999464No Hit
CGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCC473320.18285552867932817No Hit
GGGGAACCTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGG469640.1814338512823453No Hit
CCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGG465870.17997740460119707No Hit
CGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCC460310.1778294354905382No Hit
CCGGGAGGGAGGAAGACGAACGGAAGGACGGACGGCGCCGGACGCGCACG458350.17707223774649297No Hit
GGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCT453760.17529900425406053No Hit
GTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGGGACGGGGTCCGGGG453130.17505561926490315No Hit
GCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGT451520.17443363540372317No Hit
CCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGG451500.17442590889613085No Hit
GCGGGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTT444310.17164822941669966No Hit
TTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGG440440.17015315019758998No Hit
CCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCC438790.1695157133212254No Hit
CGGGCGTGGTGGTGGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGC438750.16950026030604076No Hit
GTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTT436110.16848036130385743No Hit
CGGGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTC429460.1659112975294183No Hit
GGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCC429180.1658031264231261No Hit
GACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCG425260.16428873093503568No Hit
GGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCC419370.16201327444910388No Hit
GTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGT416360.16085043505646301No Hit
GGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCC414570.1601589126269523No Hit
GTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGC408090.1576555241670477No Hit
GCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCC389780.15058190646629874No Hit
CCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACCGGCGGGCCACGGGC387320.14963154603244608No Hit
GGAAGACGAACGGAAGGACGGACGGCGCCGGACGCGCACGCCCCGCCGGG386120.14916795557690818No Hit
CGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGG384160.14841075783286298No Hit
GTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGC379150.14647526768099228No Hit
GTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCG378360.1461700706310965No Hit
GGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTCC377160.1457064801755586No Hit
GTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGG374300.14460158958985997No Hit
CGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGG372080.14374394724711487No Hit
GCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCG366720.14167324321237898No Hit
GGGGTTTCGTACGTAGCAGAGCAGCTCCCTCGCTGCGATCTATTGAAAGT365810.1413216871169294No Hit
CCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCAC364120.14066879722538023No Hit
CTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGT362580.14007385614077328No Hit
CGCGCGGCCAACCCCCGGGACGGGGACCGGCGGGCCACGGGCCCGGCTCG353700.13664328676979287No Hit
GTGGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCT348730.13472324963310678No Hit
CTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGGGACGG348710.1347155231255145No Hit
CGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGG346200.1337458464226811No Hit
GGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACC344340.13302728121659732No Hit
GGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCC343770.13280707575021683No Hit
CGGGGAGGAGGCGGGAACCGAAGAAGCGGGGCGCGCCGACCGGGGTCGCG342730.13240529735541734No Hit
GGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGAT342270.13222758768079448No Hit
CTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGGGGGGA336820.13012211436189322No Hit
TTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGG336410.1299637209562511No Hit
GGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCG333150.12870430021870652No Hit
TGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTC328000.12671472451368976No Hit
CCCGGCTCGGCGCGGCCGCCTCCGCGGCTCCCAAACCACGCTCCCCGGAC327860.12666063896054366No Hit
CGCCGACCGCGGGCGTGGTGGTGGGGGTGGGGGGGAGGGCGCGCGACCCC327120.12637475817962862No Hit
AGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCC325570.12577595384122553No Hit
GCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCC325570.12577595384122553No Hit
CCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGGGGGGAGG325520.12575663757224478No Hit
CCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCCT325440.12572573154187558No Hit
GGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTCCC325050.12557506464382576No Hit
CGGCGGTGAACGGGGAGGAGGCGGGAACCGAAGAAGCGGGGCGCGCCGAC316920.12243423930755658No Hit
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA315430.12185861449193036No Hit
GGGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCG311700.12041762082596674No Hit
CGGCGCCGGACGCGCACGCCCCGCCGGGCCCCCCGCACGCACGCGCGCGC311030.12015878282162476No Hit
CCGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGG310320.11988449180209819No Hit
CTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGGGGGGAGGGCGCGCGA310250.11985744902552513No Hit
GAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGG309490.11956384173701781No Hit
GCGGCTCGTCCGCTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGG308030.11899980668278005No Hit
GGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCC305070.11785628355911991No Hit
CCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTC291450.11259453188876486No Hit
GGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTG290870.11237046316858823No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT290390.11218502698637307No Hit
GGGAACCGAAGAAGCGGGGCGCGCCGACCGGGGTCGCGCGCCCTCCCCCC289420.11181029136814662No Hit
CCTCGACACAAGGGTTTGTCCGCGCGCGCGCGCGCGCGTGCGTGCGGGGG286930.11084834117290548No Hit
GCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGG286370.11063199896032114No Hit
CTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGGG284940.11007955366747182No Hit
GGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCC283770.10962755297332237No Hit
CCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGT283140.10938416798416499No Hit
GTTTGTCCGCGCGCGCGCGCGCGCGTGCGTGCGGGGGGCCCGGCGGGGCG280960.10854197865660449No Hit
GCGGGGCGCGCCGACCGGGGTCGCGCGCCCTCCCCCCCACCCCCACCACC277970.1073868657715559No Hit
GCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCT273290.10557886299495814No Hit
CCGGACCCCGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGG272030.10509209301664335No Hit
GGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTC269040.10393698013159479No Hit
GCGGGCGTGGTGGTGGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCG262660.10147222420965167No Hit
CGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGC261870.1011670271597559No Hit
GCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCC261740.10111680486040596No Hit
GCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGC260280.1005527698061682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCCC57300.070.48051
AGGCAGG42350.065.9456256
AACCGAC22450.061.1425485
TGCAGGT663800.060.4387741
GGCAGGA49250.056.9954157
TATCCCC70800.056.955612
GGAGGCA49900.056.8245244
GAGGCAG50450.055.9225965
ATCCCCT75400.053.480863
CGACGAA29600.052.7913638
GTCGGTT7750.052.11011
GGCGTAC21800.051.8710671
CTGTGTG74350.051.552688
TGTGTGC74900.051.1107069
CCCCTGT79400.050.3080565
CCTGTGT78450.050.2508967
TGTACTC65800.049.373312
TTGTACT66500.049.083981
GTACTCT66550.048.888263
AGGTCGA868850.047.969414