FastQCFastQC Report
Sat 24 Aug 2019
H7G5CBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7G5CBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59519148
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19386863.2572475667830463No Hit
GTATATTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAA1803960.3030890159919628No Hit
TGGCGTTAAATTCTAGGTGGAAGTCGTGCATTTATTGAAGGCCGTTTATG1474280.2476984381564064No Hit
TTTAGGGTCTCCGCATAACACCCAATTTCCATTTCCAAATTACCCTCACC1313440.22067520186948913No Hit
TTTAATGAACAAACTGCTTGATCATTTCTAGGGATTTATTGTAGTTTCGT1230560.20675027135805105No Hit
GGTTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAA825780.138741905378081No Hit
GAATTTTATTAGACCTTGAGATCTTCTTATCTGTGCAACTGATGATATTT671210.11277211159003821No Hit
TCATGCATCCAAATACAATGATCCAGGGAAAAAGACATTGAAGGGATGAA616670.10360867396824967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAGG406450.069.259411
ACGAGGT416400.068.815712
GTAAACT427750.067.675967
AGAACAG344850.067.664632
ACTGTAC345250.067.3322627
GAGAACA340300.067.315061
TGGCGTT170500.067.094661
ACAGTGT350800.067.076545
GGAGAAT235550.067.0307161
CCAGATT321350.066.9656751
TAGGATC397400.066.8373817
ACCTGGG243000.066.7588914
CCTGGGA238500.066.7127515
GTTCGAA353600.066.6053410
TGTTCGA353350.066.592929
ATAGGAT273700.066.40594516
GAGATCT114550.066.3934918
CTGGGAT239700.066.348916
CCTTGAG179600.066.31644414
AGGGTCT167350.066.2339554
Produced by FastQC (version 0.11.5)